Thermo Fisher Scientific Prohibitin Monoclonal Antibody (5H7)
Prohibitin 단백질 검출용 Mouse Monoclonal Antibody (Clone 5H7). Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat, Non-human primate 시료에 반응. 장기 보관 시 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5H7 |
| Immunogen | Purified recombinant fragment of human PHB expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11152872 |
Product Specific Information
MA5-15899 targets PHB in indirect ELISA, FACS, ICC, IHC, IF, and WB applications and shows reactivity with Human, Mouse, Non-human primate, and Rat samples.
The immunogen is a purified recombinant fragment of human PHB expressed in E. coli.
MA5-15899 detects PHB, which has a predicted molecular weight of approximately 30 kDa.
Target Information
Prohibitin is encoded by an evolutionarily conserved gene that is ubiquitously expressed. It functions as a negative regulator of cell proliferation and may act as a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer.
Prohibitin is expressed as two transcripts with varying lengths of the 3′ untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this 3′ untranslated region may function as a trans-acting regulatory RNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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