Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), eFluor 450, eBioscience
Mouse IFN gamma 인식하는 중화능 단일클론 항체로, eFluor 450 형광 표지되어 Flow Cytometry 분석에 최적화됨. Violet 레이저(405 nm)로 여기되며, 다양한 형광 및 비표지 포맷 제공. 면역조절 연구 및 세포 내 염색에 적합.
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Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 4 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 199 publications |
| ELISA | - | View 8 publications |
| Neutralization | - | View 1 publication |
| Functional Assay | - | View 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | XMG1.2 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1834367 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 561
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 660
- FITC
- Functional Grade
- PE / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
The XMG1.2 antibody reacts with mouse interferon (IFN) gamma and functions as a neutralizing antibody. Mouse IFN gamma is a 20 kDa cytokine produced by activated T, B, and NK cells, exhibiting antiviral and antiparasitic activity. It synergizes with TNF alpha to inhibit proliferation of normal and transformed cells and modulates immune responses across various cell types expressing IFN gamma receptors. Glycosylation does not affect its biological activity.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Intracellular staining and flow cytometric analysis of stimulated mouse splenocytes, effective at ≤0.5 µg/test (in 100 µL final volume). Optimal titration recommended.
Fluorochrome Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emits at 445 nm. Compatible with Violet laser detection systems.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor produced by T-lymphocytes and NK cells. It is a dimeric glycoprotein (~45 kDa) composed of two 146 amino acid subunits. IFN gamma exhibits species-specific biological activity, with human IFN gamma non-reactive to mouse. Functions include antiviral and antiproliferative activity, MHC induction, macrophage activation, and enhanced B-cell immunoglobulin secretion. It signals via the IFN gamma receptor I/II complex and activates the JAK-STAT pathway. Mutations in the IFN gamma gene are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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