Thermo Fisher Scientific Goat anti-Rabbit IgG (Heavy chain) Superclonal Secondary Antibody, Alexa Fluor Plus 405
Alexa Fluor Plus 405 형광으로 표지된 염소 유래 항-토끼 IgG (Heavy chain) 2차 항체. 높은 특이성과 로트 간 일관성 제공. 최소 교차반응으로 다양한 면역형광 및 유세포분석에 적합. 밝은 신호와 낮은 배경으로 고감도 이미징 가능.
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Thermo Fisher Scientific Goat anti-Rabbit IgG (Heavy chain) Superclonal Secondary Antibody, Alexa Fluor Plus 405
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1–8 µg/mL |
| Flow Cytometry (Flow) | 1–4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Rabbit Immunoglobulin |
| Conjugate | Alexa Fluor™ Plus 405 |
| Excitation / Emission Max | 404 / 455 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | Proprietary buffer, pH 6.5 |
| Contains | 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2921060 |
| Target | IgG (Heavy chain) |
| Antibody Form | Whole Antibody |
Additional Formats
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 647
- Alexa Fluor 680
- Alexa Fluor 790
- Alexa Fluor Plus 488
- Alexa Fluor Plus 647
- Biotin
- HRP
- Custom conjugation available upon request
Product Specific Information
Recombinant antibodies are generated using cloned genes encoding the desired antibody, expressed in vitro to ensure superior specificity and consistency between lots.
Minimal cross-reactivity is observed with mouse, rat, human, bovine, guinea pig, and donkey IgG.
Alexa Fluor Plus secondary antibodies deliver brighter fluorescence compared to conventional Alexa Fluor conjugates, enhancing signal-to-noise ratio and image clarity.
These antibodies utilize proprietary dye chemistry for improved imaging performance and sensitivity.
Usage Tip:
Centrifuge the conjugate solution briefly before use and utilize only the supernatant to minimize nonspecific background staining. Optimal dilution should be determined empirically for each application.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified and specifically recognize rabbit immunoglobulins.
They are widely used for detection, sorting, or purification of rabbit antibodies and support multiple detection systems such as HRP, AP, and fluorescence.
Secondary antibodies amplify signals by binding multiple molecules to a single primary antibody, enhancing sensitivity.
They can be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity and versatility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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