Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (MIH4), PE, eBioscience

Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (MIH4), PE, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test
Publications: View 33 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• eFluor 450
• FITC
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 645
• Request custom conjugation

Product Specific Information

The MIH4 monoclonal antibody reacts with human PD-1 (programmed death-1), a 55 kDa member of the immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes.
Two members of the B7 family, PD-L1 (B7-H1) and PD-L2 (B7-DC), serve as PD-1 ligands. These ligands have overlapping functions and are constitutively expressed on some normal tissues and upregulated on activated antigen-presenting cells.
The MIH4 antibody recognizes a different epitope than clone J105.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated on PHA-stimulated normal human peripheral blood cells
Recommended Usage: 5 µL (0.5 µg) per test (defined as staining a cell sample in 100 µL volume)
Cell Range: 10⁵–10⁸ cells/test (empirically determined)
Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Cell-mediated immune responses are initiated by T lymphocytes stimulated by peptides bound to MHC molecules on antigen-presenting cells (APC). Activated T cells express PD-1 (PDCD-1), which mediates inhibitory signals through binding to PD-L1 or PD-L2.
PD-1 signaling inhibits immune activation in the absence of danger signals such as LPS. PD-1 knockout mice exhibit hyperactivated immune systems and autoimmune diseases.
Despite its predicted molecular weight, PD-1 often migrates at a higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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