Thermo Fisher Scientific RNF20 Polyclonal Antibody
RNF20 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 인간과 생쥐에 반응합니다. 액상 형태로 제공되며 4°C에서 보관합니다.
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Thermo Fisher Scientific RNF20 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 125 and 175 of Human Ring Finger 20 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on sequence identity, antibody is predicted to react with Chicken and Bovine
Target Information
RNF20 is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. It suppresses expression of certain protooncogenes and growth-related genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS).
RNF20 positively regulates the p53 tumor suppressor and numerous histone H2A and H2B genes. Together with the deubiquitinase USP44, RNF20 modulates H2B monoubiquitination to regulate stem cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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