Thermo Fisher Scientific NUP214 Polyclonal Antibody
NUP214 단백질을 인식하는 Rabbit Polyclonal Antibody로 인간 시료에 반응합니다. Western blot 및 Immunoprecipitation에 적합하며 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며 4°C에서 보관합니다.
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Thermo Fisher Scientific NUP214 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1250 and 1300 of human Nucleoporin 214 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
The nuclear pore complex is a massive structure that spans the nuclear envelope, regulating macromolecule transport between nucleus and cytoplasm. Nucleoporins, including NUP214, are key components of this complex.
NUP214 localizes to the cytoplasmic face of the nuclear pore complex and is essential for proper cell cycle progression and nucleocytoplasmic transport.
The 3′ portion of the NUP214 gene can form a fusion gene with DEK on chromosome 6 in a t(6;9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome.
Alternative splicing generates multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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