Thermo Fisher Scientific CHK2 Monoclonal Antibody (2CHK01 (DCS-293))

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg/mg protein Lysate

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

2CHK01 (DCS-293)

Immunogen

Recombinant Chk2 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10987109

Product Specific Information

MA5-11940 targets Chk2 in immunofluorescence, immunoprecipiation, and Western blot applications and shows reactivity with Human samples. The antibody does not react with Chk1.

The MA5-11940 immunogen is recombinant Chk2 protein.

Target Information

CHEK2 (CHK2) is a serine/threonine kinase and a component of the DNA damage checkpoint pathway. A mutation in CHK2 has been linked to cancer. CHK2 is activated by DNA damage and phosphorylates several modulators of cell cycle control including tumor suppressor proteins. In addition, Chk2 can phosphorylate BRCA1, allowing BRCA1 to restore survival after DNA damage. Chk2 is a putative tumor suppressor protein, with mutations in to the Chk2 gene linked to Li-Fraumeni syndrome, a familiar cancer usually associated with inherited mutations in TP53. Also, mutations in CHK2 are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.