Thermo Fisher Scientific Bcl-xL Monoclonal Antibody (7D9)
Bcl-xL 단백질을 타겟하는 Thermo Fisher Scientific의 7D9 모노클론 항체로, WB, IHC, ICC/IF, Flow, IP 등 다양한 연구용 응용에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며, Protein A로 정제된 액상 형태로 제공됩니다. 세포 사멸 연구 및 미토콘드리아 기능 분석에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (IHC) | - | View 11 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:400 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 | - |
| Flow Cytometry (Flow) | 1 µg/test | - |
| Immunoprecipitation (IP) | 2–4 µg | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7D9 |
| Immunogen | Synthetic peptide corresponding to aa 35–50 (TEAPEETEAERETPSA) of mouse Bcl-xL protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10986767 |
Product Specific Information
MA5-11950 targets BCL-XL in FACS, IF, IHC (P), and IP applications.
This antibody can be used to stain cells by IF following fixation/permeabilization in either methanol or 4% paraformaldehyde/0.5% octyl-beta-glucoside.
The antibody can be used at a lower concentration when fixed and permeabilized by the latter method.
Target Information
BCL-XL is a member of the BCL-2 protein family, which forms hetero- or homodimers acting as anti- or pro-apoptotic regulators.
Located at the outer mitochondrial membrane, BCL-XL regulates mitochondrial membrane potential, reactive oxygen species production, and cytochrome C release—key processes in apoptosis.
Two alternatively spliced transcript variants encode distinct isoforms: the longer isoform inhibits apoptosis, while the shorter activates it.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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