Thermo Fisher Scientific Bcl-xL Monoclonal Antibody (7D9)

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:20-1:200

-

Flow Cytometry (Flow)

1 µg/test

-

Immunoprecipitation (IP)

2-4 µg

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

7D9

Immunogen

A synthetic peptide corresponding to aa 35-50 (TEAPEETEAERETPSA) of mouse Bcl-xL protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BCL-XL,uniProtId:Q64373-1,ncbiNodeId:10090,antigenRange:35-50,antigenLength:233,antigenImageFileName:MA5-11950_BCL-XL_Q64373-1_House_mouse.svg,antigenImageFileNamePDP:MA5-11950_BCL-XL_Q64373-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10986767

Product Specific Information

MA5-11950 targets BCL-XL in FACS, IF, IHC (P) and IP applications.

MA5-11950 can be used to stain cells by IF following fixation/permeabilization in either methanol or 4% paraformaldehyde/0.5% octyl-beta-glucoside. The antibody can be used at a lower concentration when fixed and permeabilized by the latter method.

Target Information

BCL-XL is a part of the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BCL-XL is located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential and controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.