Thermo Fisher Scientific Biotin Polyclonal Antibody, Rhodamine
Biotin에 특이적인 Rabbit Polyclonal Antibody로 Rhodamine 형광 표지됨. Western blot, ICC/IF, ELISA 등 다양한 응용에 사용 가능. Lyophilized 형태로 안정적이며, 573/591 nm 형광 특성 제공. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:500–1:2,500
ELISA
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Biotin conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Rhodamine |
| Excitation / Emission Max | 573 / 591 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Biotin Antibody Texas Red™ Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion exchange chromatography, followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotinylated IgG, and Biotinylated Bovine Serum Albumin.
Target Information
Biotin is a water-soluble B complex vitamin (Vitamin B7). It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species.
Biotin is essential for incorporation of carbon dioxide into organic compounds and has a molecular weight of approximately 244 Da.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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