Thermo Fisher Scientific Biotin Polyclonal Antibody, Rhodamine

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:500-1:2,500

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Conjugate

Rhodamine Rhodamine Rhodamine

Excitation/Emission Max

573/591 nm View spectra

Form

Lyophilized

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Biotin Antibody Texas Red™ Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotinylated IgG and Biotinylated Bovine Serum Albumin.

Target Information

Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7. It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required for incorporation of carbon dioxide into organic compounds and exhibits a MW of ~244 Da.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.