Thermo Fisher Scientific CD8a Monoclonal Antibody (YCATE55.9), eFluor 450, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (YCATE55.9), eFluor 450, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test
• Publications: View 2 publications

Product Specifications

Available Formats

• APC
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

This YCATE55.9 monoclonal antibody reacts with canine CD8 alpha. CD8α is expressed as a heterodimer (CD8α/β) or homodimer (CD8α/α) and found on thymocytes, mature T cells, NK cells, and intestinal intraepithelial lymphocytes (IELs). CD8 binds to MHC class I and plays a role in thymocyte development and mature T cell activation.

This antibody has been reported to inhibit cytotoxic T cell generation and function in vitro and to be immunosuppressive in vivo.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested on canine peripheral blood cells
• Recommended Use: 5 µL (0.5 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test)

eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emits at 445 nm. Ensure instrument compatibility.

• Excitation: 405 nm
• Emission: 445 nm
• Laser: Violet laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin family. It acts as a co-receptor with TCR for MHC class I molecules and is essential for T cell activation and differentiation. CD8 is expressed on T cells, NK cells, monocytes, and dendritic cells. Engagement with MHC-I/peptide complexes triggers Lck activation, leading to lymphokine production and cytotoxic T lymphocyte (CTL) activation. Activated CTLs play a key role in pathogen and tumor clearance. Differentiation is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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