Thermo Fisher Scientific VEGF-A Polyclonal Antibody
Bovine VEGF-A 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, ELISA, IP에 사용 가능. Protein A로 정제된 Lyophilized 형태이며, 다양한 종과 교차 반응 가능. 혈관 신생 연구 및 VEGF 관련 신호전달 연구에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:500 | - |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | 1:333 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein raised in yeast corresponding to the 164 amino acids of the mature bovine VEGF-A protein. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This antibody is specific for bovine VEGF-A protein.
A BLAST analysis suggests cross-reactivity with VEGF-A from bovine, pig, sheep, and macaque (100% homology).
Partial reactivity is expected with human, horse, dog, cat, or guinea pig (95% homology), and rat or mouse (93% homology).
Cross-reactivity with other species has not been determined.
Target Information
Vascular Endothelial Growth Factor (VEGF) is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis and tumor progression.
VEGF promotes endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability.
The VEGF family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF.
VEGF signaling via receptors FLT1 (VEGFR-1) and FLK1/KDR (VEGFR-2) is essential for embryonic vasculature development.
Elevated VEGF levels are associated with POEMS syndrome (Crow-Fukase syndrome), affecting multiple organs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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