Thermo Fisher Scientific ATF1 Recombinant Rabbit Monoclonal Antibody (JJ207-04)
ATF1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry에 적합합니다. 높은 특이성과 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션으로 제조되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JJ207-04 |
| Immunogen | Synthetic peptide within Human ATF1 (aa 222–271) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809717 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select the best candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
The ATF1 gene encodes an activating transcription factor belonging to the ATF subfamily and bZIP family. It regulates expression of downstream genes related to cellular growth, survival, and other functions.
Phosphorylation at serine 63 by various kinases enhances transactivation and transcriptional activity, contributing to cell transformation.
Chromosomal translocations involving ATF1 and FUS (chromosome 16) or EWSR1 (chromosome 22) generate chimeric proteins observed in angiomatoid fibrous histiocytoma and clear cell sarcoma.
A pseudogene exists on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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