Thermo Fisher Scientific CHD4 Polyclonal Antibody
CHD4 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF, ELISA 등에 사용 가능. Human, Mouse, Rat에 반응하며, Affinity Chromatography로 정제됨. PBS/glycerol buffer에 보관하며 -20°C에서 안정적. 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1520–1690 of human CHD4 (NP_0012642) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.78 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804694 |
Product Specific Information
Immunogen sequence:
ELAEVEENKK MSQPGSPSPK TPTPSTPGDT QPNTPAPVPP AEDGIKIEEN SLKEEESIEG EKEVKSTAPE TAIECTQAPA PASEDEKVVV EPPEGEEKVE KAEVKERTEE PMETEPKGAA DVEKVEEKSA IDLTPIVVED KEEKKEEEEK KEVMLQNGET PKDLNDEKQK K
Positive Samples: HeLa, Jurkat, 293T, HT-1080
Cellular Location: Cytoplasm, Nucleus, Centrosome, Cytoskeleton, Microtubule Organizing Center
Target Information
The CHD4 gene encodes a protein belonging to the SNF2/RAD54 helicase family. It is the main component of the nucleosome remodeling and deacetylase (NuRD) complex, playing a crucial role in epigenetic transcriptional repression. Antibodies against this protein are often found in patients with dermatomyositis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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