Thermo Fisher Scientific 53BP1 Polyclonal Antibody, DyLight 488
DyLight 488 형광 표지된 53BP1 폴리클로날 항체로, Western blot, IHC, ICC, Flow cytometry에 사용 가능. 사람 및 마우스 시료 반응. 항원 친화 크로마토그래피로 정제되었으며, 4°C 암조건에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide representing a portion of human tumor protein p53-binding protein 1 encoded in exon 11, 12, and 19 (LocusLink ID 7158). |
| Conjugate | DyLight™ 488 |
| Excitation/Emission Max | 492/519 nm |
| Form | Liquid |
| Concentration | 0.64 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154654 |
Product Specific Information
This antibody reacts with Human samples in Western blot and ICC applications, and with Mouse in ICC applications.
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to presumptive sites of damage, specifically double-strand breaks. It may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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