Thermo Fisher Scientific CD23 Monoclonal Antibody (EBVCS2), PE-eFluor 610, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• Publications: View 1 publication

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant UV 805
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The EBVCS2 monoclonal antibody reacts with human CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells, and at low levels on T, NK, Langerhans cells, and platelets. Expression is upregulated upon B cell activation, and soluble forms are biologically active. CD23 acts as a low affinity receptor for IgE and regulates IgE response and B cell activation. CD21 and CD11b/CD11c α-subunits bind to CD23.

• Applications Reported: Flow cytometry
• Applications Tested: Pre-diluted and tested on normal human peripheral blood cells
  • Use 5 µL (0.25 µg) per test (100 µL final volume)
  • Suitable for 10⁵–10⁸ cells/test

Fluorophore Information:
PE-eFluor 610 is excited by 488–561 nm lasers and emits at 607 nm. Recommended filter: 610/20 band pass (equivalent to PE-Texas Red). Ensure instrument compatibility.

Light Sensitivity:
Sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation: 488–561 nm
Emission: 607 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers

Target Information

CD23 is a 45 kDa glycoprotein present on a subpopulation of peripheral blood and tonsil B cells and highly expressed on EBV-transformed B lymphoblasts. It is identical to the low-affinity IgE receptor on B cells. Expression has been detected in B cell chronic lymphocytic leukemia and some lymphomas. Functionally, CD23 regulates B cell growth, differentiation, and IgE production. Soluble CD23 acts as a mitogenic factor. CD23 dysfunction is associated with chronic conjunctivitis and chronic lymphocytic leukemia.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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