Countstar Mira BF

Brightfield Cell Analyzer

The Countstar Mira BF Automated Cell Analyzer integrates advanced optical imaging technology, intelligent image recognition, and user-friendly consumable design to achieve the detection and analysis of multiple samples. Results are generated in seconds and provide you with a full range of cell culture information that may assist in determining ideal cell culture conditions.

Core Advantages

• Future development of customized adapters may allow the analysis of more culture vessels.
• Innovative, optical zoom technology will meet the needs of many researchers who want to analyze a wide range of cell species with a single instrument.
• The Al based, self-learning imageanalysis algorithms are capable of accurately identifying and quantifying small aggregated suspension cells, organoids, and a wide variety of adherent cells.

Product Details

Adapters for Your Favorite Consumables

The Countstar Mira BF Automated Cell Analyzer analyzes cells and particles using our proprietary Countstar chamber slide. By switching to one of the alternative adapter plates, a standard hemocytometer of type Neubauer, Thoma, or Fuchs-Rosenthal can be used as sample carriers. With these adapters, the Countstar Mira BF is also compatible with 35/60/100mm cell culture petri dishes and T25 mini cell culture flasks. The sliding adapter allows also the non- invasive, repeated analysis of 4-, 8-, 12-, or 18 chamber µ-slides for monitoring adherent cells, organoids, and 3D cell spheres during their cultivation. Future development of customized adapters may allow the analysis of more culture vessels.

Compatible With Multiple Types of Consumables
Countstar Chamber Slides Application:Organoid Analysis/Cell and Object counting/Trypan Blue Viability Assays	3.5/6/10cm Petri Dishs Applications: Organoid Analysis/Confluence Analysis	** T25 Mini Cell** Culture Flasks Applications: Confluence Analysis
Hemocytometer Applications: Trypan Blue Viability/Cell Counting	8-Chamber µ-Slides Applications: Organoid Analysis/Cell Confluence Studies	4-Chamber µ-Slides Applications: Organoid Analysis/Cell Confluence Studies

Innovative, Optical Zoom Technology (5.0x, 6.6x, 8.0x)

The Countstar Mira BF Automated Cell Analyzer uses the easily adjustable Optical Zoom Technology, which allows analysis of objects/cells in a range of 2µm up to 180µm. This flexibility will meet the needs of many researchers who want to analyze a wide range of cell species with a single instrument.

– Cross linked agarose beads (approx. 45µm Ø) in 5.0x, 6.6x, and 8.0x magnification

Pre-installed and Customizable BioApps Offering a Variety of Analytical Assay

The layout of the Android-based Graphical User Interface (GUI) of the Countstar Mira BF allows for a simple and efficient user experience. A number of preset and customizable BioApps (assay templates), fitted to a variety of cell types, can be added and modified to meet current and future research goals. Using a BioApp requires a simple click of the desired BioApp, naming the sample, selecting the dilution, and a test can be performed, making the analysis simple and convenient. The design of the GUI streamlines any experiment and provides immediate and detailed access to the acquired hi-res images and multiple result data.

– Analyze various organisms using versatile BioApps(assay templates)

– Selection of BioApps

AI-based Image Recognition Algorithms

The Al (Artificial Intelligence) based, self-learning image analysis algorithms of the Countstar Mira BF are capable of accurately identifying and quantifying small aggregated suspension cells, organoids, and a wide variety of adherent cells. Beyond that, the Countstar Mira BF offers a wide range of analysis functions by providing the ability to analyze organoids, 3D cell spheres, and other, 2-180µm large dimension particles. The powerful imaging capabilities of the Countstar Mira BF allow customized solutions based on future needs.

**Result Output, Data Storage, and Export

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The intuitive software of the Countstar Mira BF gives an easy access to all acquired images and result data. Sorted in the Assay (BioApp) data library by date and time, a selection of the desired experiment is done by a few clicks on the touch screen. The internal storage capacity of 128GB enable the operator to store up to 50,000 tests.

-Overview menu showing results of a single analysis

Beyond standard results such as cell concentration,viability (mortility) rate, diameter, roundness, and agglomeration rate, the Countstar Mira BF can determine and display the cell culture confluence status, the rate of adherent cells, the number of organoids, their sizes, and their central light transmittance. Diagrams, generated inside the software, will visualize the results of the experiments.

-Automatically generated diameter distribution histogram of a selected sample

Analytical tools and the hi-res images visualize the cell growth status and the cell size distribution to optimize cell culture conditions. Export tools for images, csv data, and pdf reports are integrated for an advanced experiment analysis.

-Comparison of growth curves of two similar cultivation experiments

** Fast and Intuitive Handling**

The experiment preparation menu: Select one or more chambers, enter the corresponding sample ID, define the used dilution and click on Start – a convenient and time-saving analysis experience, completedin less than 20 sec.

-Test Preparation Menu

The consumables management library allows a quick selection of the appropriate sample carrier for a BioApp. The focus data for the consumables are already pre-registered. New consumables with different geometry can be added to this library, and their focus layer value can be easily determined in the Manual Analysis menu.

-Consumable Management

The preset experiment protocols in the BioApp library do not require any user interaction during the analysis. Up to five chambers can be analyzed consecutively. Up to three (3) regions of interest can be acquired in a single chamber of a Countstar Chamber slide to optimize the assays’s accuracy and precision.