Thermo Fisher Scientific SF1 Polyclonal Antibody
SF1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF에 사용 가능. 고순도(>95%) 항원 친화 크로마토그래피 정제. 68 kDa 내인성 단백질 검출용 연구용 시약.
- 판매단위
- pk
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 45–98 of human SF1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553370 |
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 68 kDa.
Purity is >95% by SDS-PAGE.
Target Information
Splicing factor 1 (SF1) is a nuclear pre-mRNA splicing factor. It specifically recognizes the intron branch point sequence at the 3′ splice site, together with U2 auxiliary factor (U2AF), and is essential for early spliceosome assembly. SF1 also contributes to nuclear pre-mRNA retention and transcriptional repression. The protein contains multiple functional domains including:
- N-terminal U2AF ligand motif
- Central hnRNP K homology motif and quaking 2 region
- Zinc knuckles domain
- C-terminal proline-rich domain
Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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