Thermo Fisher Scientific Phospho-MNK1 (Thr197, Thr202) Polyclonal Antibody
MNK1 단백질의 Thr197, Thr202 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot 및 ELISA에 적용 가능하며, 인간, 마우스, 랫트 반응성. 액상 형태로, PBS/glycerol 버퍼에 보관하며 -20°C에서 안정적. 연구용으로만 사용.
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Thermo Fisher Scientific Phospho-MNK1 (Thr197, Thr202) Polyclonal Antibody
Applications and Tested Dilution
- Western Blot (WB): 1:500–1:2,000
View 1 publication - ELISA: 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphorylated peptide around T197 & T202 of rat MKNK1 (NP_0010377321) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2855548 |
Product Specific Information
- Immunogen sequence: ITTPELTT
Target Information
The MAP kinase-interacting kinases (MKNK1 and MKNK2) are activated by MAP kinases and regulate protein synthesis by phosphorylating eIF4E on Ser209, potentially contributing to tumorigenesis.
Overexpression of Mnk2 increases phosphorylation of endogenous eIF-4E, acting as an eIF-4E kinase in vivo.
Mnk2 may participate in responses to environmental stress and cytokines.
Active mutants of MNK1 and MNK2 reduce cap-dependent translation relative to cap-independent translation in 293 cells.
Human Mnk2 is ~94% identical to murine Mnk2 and ~71% identical to human Mnk1.
In vitro studies show Mnk2 is a better substrate than Mnk1 for Erk2, p38MAPKα, and p38MAPKβ.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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