Thermo Fisher Scientific PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Monoclonal Antibody (PC10)
PCNA 단백질을 인식하는 단클론 항체로, G1 및 S기 세포의 증식 마커로 사용됩니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. 다종 반응성이 뛰어나며, 고순도의 Protein A/G 정제 항체입니다.
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- 5111-MSM1-P1ABX
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Chicken, Fruit fly, Human, Mouse, Non-human primate, Pig, Rat, Yeast, Zebrafish |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PC10 |
| Immunogen | Rat PCNA/protein A fusion protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
PCNA (Proliferating Cell Nuclear Antigen) is a polymerase delta auxiliary protein essential for DNA replication. It plays a key role in DNA excision and mismatch repair pathways.
PCNA interacts with CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
It serves as a marker for cells with proliferative potential and is widely used to assess tumor proliferation status.
PCNA is localized in the nucleus, functions as a homotrimer, and enhances DNA polymerase delta processivity during leading strand synthesis.
Upon DNA damage, PCNA undergoes ubiquitination and participates in the RAD6-dependent DNA repair pathway.
Two transcript variants encoding the same protein have been identified, and pseudogenes exist on chromosome 4 and the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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