Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), Alexa Fluor 488, eBioscience
인간 CD107a(LAMP-1)을 인식하는 eBioH4A3 단클론 항체로, Alexa Fluor 488 형광표지되어 있습니다. Flow cytometry 및 세포 내 염색에 적합하며, 499/520 nm에서 여기/방출됩니다. 4°C에서 암소 보관하며 냉동 금지. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 0.125 µg/mL | View 10 publications |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 13 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioH4A3 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2016585 |
Additional Available Formats
- Unconjugated
- Brilliant Violet 421
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated ~110 kDa protein expressed mainly in lysosomal/endosomal membranes across most cell types. It is transiently expressed on the surface of degranulating cytolytic T cells and is upregulated on activated platelets and certain cancer cells.
Applications Reported:
- Intracellular staining followed by flow cytometric analysis
- Surface staining in flow cytometric-based degranulation assays
Applications Tested:
- Pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells
- Recommended: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
Fluorescence Properties:
- Excitation: 488 nm
- Emission: 519 nm
- Laser: Blue Laser
Filtration:
0.2 µm post-manufacturing filtered
Target Information
LAMP1 (CD107a, lysosome-associated membrane protein-1) is a major lysosomal membrane component. Approximately 1–2% of total CD107a is also found on the plasma membrane. It contains a signal peptide, 18 N-linked glycosylation sites, a single membrane-spanning region, and a short cytosolic tail.
LAMP proteins are essential for lysosome biogenesis and lysosome-phagosome fusion. Upon activation, LAMP1 translocates to the plasma membrane via a tyrosine-based motif (YXXI). Disruption of this motif prevents lysosomal localization.
Surface LAMP1 promotes adhesion of PBMCs to vascular endothelium, suggesting a role in inflammation. Increased LAMP1 expression is observed in neurons and glial cells near senile plaques in Alzheimer’s disease and in macrophages and lymphocytes during thymic involution. LAMP1 serves as a marker for mast cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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