Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• Unconjugated
• Brilliant Violet 421
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• eFluor 660
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated ~110 kDa protein expressed mainly in lysosomal/endosomal membranes across most cell types. It is transiently expressed on the surface of degranulating cytolytic T cells and is upregulated on activated platelets and certain cancer cells.

Applications Reported:

• Intracellular staining followed by flow cytometric analysis
• Surface staining in flow cytometric-based degranulation assays

Applications Tested:

• Pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells
• Recommended: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Fluorescence Properties:

• Excitation: 488 nm
• Emission: 519 nm
• Laser: Blue Laser

Filtration:
0.2 µm post-manufacturing filtered

Target Information

LAMP1 (CD107a, lysosome-associated membrane protein-1) is a major lysosomal membrane component. Approximately 1–2% of total CD107a is also found on the plasma membrane. It contains a signal peptide, 18 N-linked glycosylation sites, a single membrane-spanning region, and a short cytosolic tail.
LAMP proteins are essential for lysosome biogenesis and lysosome-phagosome fusion. Upon activation, LAMP1 translocates to the plasma membrane via a tyrosine-based motif (YXXI). Disruption of this motif prevents lysosomal localization.
Surface LAMP1 promotes adhesion of PBMCs to vascular endothelium, suggesting a role in inflammation. Increased LAMP1 expression is observed in neurons and glial cells near senile plaques in Alzheimer’s disease and in macrophages and lymphocytes during thymic involution. LAMP1 serves as a marker for mast cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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