Thermo Fisher Scientific Cyclooxygenase 2 Polyclonal Antibody
Cyclooxygenase 2 (COX2)에 특이적인 Rabbit Polyclonal Antibody로, Human, Mouse, Rat 시료에 반응합니다. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 적합하며, Protein A로 정제된 고순도 항체입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from mouse Cyclooxygenase 2, amino acids 501–604 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including inflammatory prostaglandins production. The conversion involves two reactions:
- A cyclooxygenase (COX) reaction converting arachidonate to prostaglandin G2 (PGG2).
- A peroxidase reaction reducing PGG2 to prostaglandin H2 (PGH2).
COX2 is constitutively expressed in tissues such as endothelium, kidney, and brain under physiological conditions and is upregulated under pathological conditions like cancer and inflammation. Its up-regulation is associated with increased cell adhesion, phenotypic changes, resistance to apoptosis, and tumor angiogenesis. In cancer cells, COX2 contributes to prostaglandin E2 (PGE2) production, influencing motility, proliferation, and apoptosis resistance.
COX2 activity is naturally inhibited by calcitriol (active Vitamin D). Glucocorticoids repress PTGS2 gene activity by interfering with transcription initiation and elongation. COX2 is a known target of NSAIDs such as aspirin, which reduce pain and inflammation mediated by COX2.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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