Thermo Fisher Scientific MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Biotin, eBioscience

Thermo Fisher Scientific MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Brilliant UV 496, 661, 737
• Brilliant Violet 421, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 510, 610-70S, 660-120S
• NovaFluor Red 685, 710
• NovaFluor Yellow 570, 590, 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

The M5/114.15.2 monoclonal antibody reacts with mouse major histocompatibility complex (MHC) class II glycoproteins encoded by both I-A and I-E subregions (I-A^b, I-A^d, I-A^q, I-E^d, I-E^k; not I-A^f, I-A^k, or I-A^s).
It detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying H-2^b, H-2^d, H-2^q, H-2^p, H-2^r, and H-2^u haplotypes, but not H-2^s or H-2^f.
M5/114 mAb inhibits I-A–restricted T cell responses in certain haplotypes (H-2^b, H-2^d, H-2^q, H-2^u).

Applications Reported: Flow cytometry, immunohistochemistry, and immunoprecipitation.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.125 µg/test (100 µL final volume). Optimal titration recommended per assay.

Target Information

MHC class II molecules are transmembrane glycoproteins expressed on professional antigen-presenting cells (macrophages, dendritic cells, B cells).
They bind endocytosed exogenous antigens and present them to T cells. The antigen-binding groove between MHC class II α and β chains is open at both ends and accommodates peptides 15–24 amino acids long.

HLA and MHC antibodies are essential in Immunopeptidomics, enabling identification and characterization of neoantigens via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.