Thermo Fisher Scientific MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Biotin, eBioscience
마우스 MHC Class II (I-A/I-E) 항원을 인식하는 단클론 항체로, Biotin으로 결합되어 있습니다. Flow cytometry, IHC, IP 등 다양한 면역분석에 사용 가능하며, 높은 특이성과 재현성을 제공합니다. 4°C에서 보관하며, 연구용으로만 사용됩니다.
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Thermo Fisher Scientific MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (IHC) | Assay-dependent | View 10 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunohistochemistry - Free Floating (IHC (Free)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.015 µg/test | View 83 publications |
| Immunoprecipitation (IP) | Assay-dependent | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M5/114.15.2 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_529608 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 700
- APC, APC-eFluor 780
- Brilliant UV 496, 661, 737
- Brilliant Violet 421, 605, 650, 711, 786
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue 510, 610-70S, 660-120S
- NovaFluor Red 685, 710
- NovaFluor Yellow 570, 590, 660
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available
Product Specific Information
The M5/114.15.2 monoclonal antibody reacts with mouse major histocompatibility complex (MHC) class II glycoproteins encoded by both I-A and I-E subregions (I-A^b, I-A^d, I-A^q, I-E^d, I-E^k; not I-A^f, I-A^k, or I-A^s).
It detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying H-2^b, H-2^d, H-2^q, H-2^p, H-2^r, and H-2^u haplotypes, but not H-2^s or H-2^f.
M5/114 mAb inhibits I-A–restricted T cell responses in certain haplotypes (H-2^b, H-2^d, H-2^q, H-2^u).
Applications Reported: Flow cytometry, immunohistochemistry, and immunoprecipitation.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.125 µg/test (100 µL final volume). Optimal titration recommended per assay.
Target Information
MHC class II molecules are transmembrane glycoproteins expressed on professional antigen-presenting cells (macrophages, dendritic cells, B cells).
They bind endocytosed exogenous antigens and present them to T cells. The antigen-binding groove between MHC class II α and β chains is open at both ends and accommodates peptides 15–24 amino acids long.
HLA and MHC antibodies are essential in Immunopeptidomics, enabling identification and characterization of neoantigens via LC-MS/MS.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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