Thermo Fisher Scientific Rat IgG1 kappa Isotype Control (eBRG1), Brilliant Ultra Violet 563, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 570, 615, 660
• FITC
• Functional Grade
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

• The monoclonal rat IgG1 kappa is useful as an isotype control immunoglobulin.
• Reported for use in intracellular staining followed by flow cytometric analysis.
• Tested by flow cytometric analysis of normal human peripheral blood and mouse splenocytes.
• Use at the same concentration as the experimental antibody.
• Brilliant Ultra Violet™ 563 (BUV563) emits at 564 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser.
• When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
• Light sensitivity: Protect from light to avoid photo-induced oxidation.
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark.
• Excitation: 350 nm; Emission: 564 nm; Laser: Ultraviolet Laser.
• BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies produced by rats in response to antigens.
Commonly used as a negative control in experiments due to low cross-reactivity with mammalian antigens.
Applications include immunohistochemistry, flow cytometry, and western blotting.
Gene targeting in rats enables generation of disease models for biomedical research.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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