Thermo Fisher Scientific MEK2 C-Term Polyclonal Antibody, FITC
FITC로 표지된 MEK2 C-말단 폴리클로날 항체로, 인간·마우스·랫트 시료에 반응합니다. Western blot과 ELISA에 적합하며, 친화 크로마토그래피로 정제되었습니다. 498/517 nm 형광 특성을 가지며 연구용으로 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-MEK2 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH. |
| Conjugate | FITC (Fluorescein) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This fluorescein-conjugated affinity-purified antibody is directed against human MEK2 protein. Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase that plays a critical role in mitogen growth factor signal transduction. MEK2 (MAPKK2) is part of a family of tyrosine/threonine protein kinases that activate ERK1, ERK2, and MAPK enzymes by phosphorylating residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and MEK2 are activated by dual phosphorylation on serine 218 and 222 in the activation loop. Threonine 292 of MEK1 is phosphorylated by ERK2, serving as a negative feedback loop by suppressing MEK1 activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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