Thermo Fisher Scientific Transferrin Polyclonal Antibody, HRP
HRP로 결합된 Rabbit Polyclonal Anti-Transferrin 항체로 Western blot, IHC, ELISA, IP 등 다양한 응용 가능. 인간 Transferrin에 특이적이며 높은 순도(affinity chromatography)로 정제됨. 연구용으로 철 운반 관련 단백질 분석에 적합.
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Thermo Fisher Scientific Transferrin Polyclonal Antibody, HRP
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:3,000–1:12,000 |
| Immunoprecipitation (IP) | 1:300–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Human Transferrin |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if solution is not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This reagent was purified from pooled rabbit sera immunized with Human Transferrin, conjugated to horseradish peroxidase (HRP), and purified by affinity chromatography followed by exhaustive dialysis.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, purified Human Transferrin, and Human Serum.
Target Information
Transferrins (Tf) are approximately 80 kDa blood plasma glycoproteins synthesized by the liver. They bind two Fe(3+) ions along with an anion, typically bicarbonate, and are responsible for iron transport from absorption and degradation sites to storage and utilization sites.
Transferrin is the primary blood iron transport protein; about one-third of total transferrin normally contains bound iron.
Measurement of transferrin levels indicates iron-carrying capacity and abnormalities such as anemia, iron overload, or haemochromatosis. Serum transferrin may also play a role in stimulating cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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