Thermo Fisher Scientific Tryptophan 5-hydroxylase 1, Tryptophan 5-hydroxylase 2 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Target Information

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001).
In mammals, serotonin biosynthesis occurs predominantly in neurons originating in the Raphe nuclei of the brain, while melatonin synthesis occurs in the pineal gland.
TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland.
The activity of TPH is enhanced by phosphorylation via cAMP-dependent protein kinase (PKA) and Ca²⁺/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996).
Both PKA and CAM K II phosphorylate Ser58 within the regulatory domain of TPH (Kuhn et al., 1997).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.