Invitrogen™

pBAD/TOPO™ ThioFusion™ Expression Kit

The pBAD/TOPO™ ThioFusion™ Expression Kit is designed for one-step cloning and regulated expression of thioredoxin fusion proteins in E. coli(Figure자세히 알아보기

The pBAD/TOPO™ ThioFusion™ Expression Kit is designed for one-step cloning and regulated expression of thioredoxin fusion proteins in E. coli(Figure 1). The pBAD/Thio-TOPO™ vector encodes His-Patch thioredoxin as an N-terminal fusion partner. The thioredoxin fusion can significantly increase the solubility of many difficult-to-express proteins and improve the yield of protein production. The vector includes the following additional features (Figure 2):

• The araBAD promoter for tightly regulated expression in E. coli I-activated vector for 5-minute TOPO™ Cloning of _Taq_-amplified PCR products
• C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody
• C-terminal V5 epitope for detection with an Anti-V5 Antibody
• Enterokinase cleavage site for efficient cleavage of N-terminal fusion tag with EKMax™ Enterokinase

사양

구성 또는 유도성 시스템Inducible

유도제Arabinose

프로모터araBAD

제품 유형Lentiviral Expression Kit

선택 제제(진핵)None

항생제 내성 박테리아Ampicillin (AmpR)

박테리아 또는 효모 균주TOP10

분열EK (Enterokinase) Recognition Site

발현 시스템E. coli

발현 메커니즘Cell-Based Expression

단백질 태그His Tag (6x), V5 Epitope Tag, Thioredoxin

클로닝 방법TOPO-TA

수량20 reactions

벡터pBAD

제품라인TOPO™, ThioFusion™

Unit SizeEach