Invitrogen™

Vivid Colors™ pcDNA™6.2/C-EmGFP-GW/TOPO™ Mammalian Expression Vector

The Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO™ Expression Vectors (Figure 1) allow you to rapidly clone your gene and fuse자세히 알아보기

The Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO™ Expression Vectors (Figure 1) allow you to rapidly clone your gene and fuse it to the widely used and well characterized Fluorescent Proteins (FPs) from the jellyfish Aequorea victoria (1, 2). These powerful TOPO™ cloning vectors contain the Emerald Green Fluorescent Protein (EmGFP) or the Yellow Fluorescent Protein (YFP) for simple, non-invasive detection of recombinant protein (Figure 2). Both FPs have been humanized for optimal mammalian expression (3). The Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO™ Expression Vectors offer:
• Topoisomerase I for one-step, 5-minute TOPO™ cloning of your PCR-amplified gene of interest
• CMV promoter for high-level expression of the recombinant fluorescent fusion protein
• Ability to fuse EmGFP or YFP to the N- or C-terminus of your protein
• Bsd resistance marker for rapid selection of stable cell lines

사양

구성 또는 유도성 시스템Constitutive

배달 유형Transfection

프로모터CMV

제품 유형Mammalian Expression Vector

리포터 유전자GFP (EmGFP)

선택 제제(진핵)Blasticidin

단백질 태그GFP (EmGFP)

클로닝 방법TOPO-TA

수량20 reactions

벡터pcDNA

제품라인Gateway™, TOPO™, Vivid Colors™, pcDNA™

용도(애플리케이션)Reporter Assays, Subcellular Localization

Unit SizeEach