Thermo Fisher Scientific PKC alpha Recombinant Rabbit Monoclonal Antibody (ARC0197)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Positive Samples: HeLa, Jurkat, Mouse brain, Mouse lung, Rat brain
• Immunogen sequence:
  MTKHPAKRLG CGPEGERDVR EHAFFRRIDW EKLENREIQP PFKPKVCGKG AENFDKFFTR GQPVLTPPDQ LVIANIDQSD FEGFSYVNPQ FVHPILQSAV

Target Information

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity.

PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters.

Each member of the PKC family has a specific expression profile and distinct cellular roles. PKC alpha has been reported to participate in processes such as cell adhesion, transformation, cell cycle checkpoint regulation, and cell volume control. Knockout studies in mice suggest it is a key regulator of cardiac contractility and Ca²⁺ handling in myocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.