Thermo Fisher Scientific H4K20me1 Polyclonal Antibody
Western blot, ICC/IF, Peptide array에 사용 가능한 H4K20me1 폴리클로날 항체. Rabbit IgG 기반으로 히스톤 H4의 Lys20 단일 메틸화 형태를 인식. 높은 특이성과 재현성을 제공하며, 연구용으로 적합.
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Thermo Fisher Scientific H4K20me1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:250 |
| Peptide Array (Array) | 1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the amino terminus of histone H4 in which Lys20 is mono-methylated |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 46.7 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10984601 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with mono-, di-, or tri-methylated histone H3 at Lys4, Lys9, Lys27, or Lys36.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes.
The linker histone H1 interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher-order structures.
This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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