Thermo Fisher Scientific NFkB p50 Polyclonal Antibody
NFkB p50 단백질을 검출하기 위한 Thermo Fisher Scientific의 폴리클로날 항체. Western blot과 IHC(P)에서 사용 가능. 인간 시료 반응성, rabbit IgG 호스트. 액상 형태로 안정적이며 장기 보관 시 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to the internal segment of human NFkB p50 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: All human tissue
- Cellular location: Nucleus, Cytoplasm
Target Information
NFkB is a nuclear transcription factor activated by various extracellular and intracellular stimuli such as cytokines, UV radiation, stress, injury, and bacterial or viral products. It regulates cellular events including growth, differentiation, proliferation, apoptosis, and inflammation.
NFKB1 (p50) is a 50 kDa functional subunit of NFkB and a member of the Rel protein family. It is synthesized as a p105 precursor containing an N-terminal RHD region with nuclear localization, DNA-binding, and dimerization domains. NFKB1 (p50) forms homodimers or heterodimers with p65 to create the functional NFkB complex.
NFkB is mainly cytoplasmic in its inactive form bound to I-kappa-B. Upon phosphorylation of I-kappa-B, the complex translocates to the nucleus to modulate gene expression. Phosphorylation of p50 at Ser-337 enhances DNA binding efficiency. NFkB plays roles in AIDS, cancer metastasis, and rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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