Thermo Fisher Scientific Mouse anti-Rat IgG1 Secondary Antibody, APC, eBioscience

Thermo Fisher Scientific Mouse anti-Rat IgG1 Secondary Antibody, APC, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 0.25 µg/test

Product Specifications

Additional Formats

• Biotin
• eFluor 615
• FITC
• PE
• Request custom conjugation

Product Specific Information

The R1-12D10 monoclonal antibody recognizes rat IgG1 antibodies and is used as a secondary reagent in flow cytometry and microscopy.
It does not cross-react with other rat isotypes or mouse IgG1.
Tested for flow cytometric analysis of cells stained with a rat IgG1 primary antibody.
Recommended usage: ≤ 0.25 µg/test (100 µL final volume).
Cell number can range from 10⁵ to 10⁸ cells/test and should be titrated for optimal performance.
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Anti-Rat secondary antibodies are affinity-purified with well-characterized specificity for rat immunoglobulins.
They are useful for detection, sorting, or purification of their specified targets.
Secondary antibodies provide versatility and signal amplification, enabling use in multiple detection systems (HRP, AP, fluorescence).
Commonly produced by immunizing host animals with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, fragmentation, label conjugation).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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