Thermo Fisher Scientific p53R2 Polyclonal Antibody
p53R2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 적합합니다. Human, Mouse, Rat 시료에 반응하며, 세포 내 p53 신호전달 및 DNA 손상 복구 연구에 유용합니다. 액상 형태로 1 mg/mL 농도, 4°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein within C-terminal human p53R2 (aa 200–351) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | TBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2913443 |
Product Specific Information
- Positive Control: Rat skeletal muscle tissue lysate, mouse skeletal muscle tissue lysate, mouse cerebellum tissue lysate, human skeletal muscle tissue lysate, Daudi cell lysate, mouse colon tissue, rat cerebellum tissue, SH-SY5Y.
- Subcellular Location: Cytoplasm, Nucleus.
Target Information
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including p53R2, Chk2, p53AIP1, Noxa, PIDD, and PID/MTA2, have been discovered.
p53R2 is a p53-inducible gene containing a p53 binding sequence and encodes a subunit of the enzyme ribonucleotide reductase. It is induced by ultraviolet and gamma irradiation that cause DNA damage. The p53R2 gene product is directly involved in the p53 checkpoint for DNA repair. The p73 isoform of the p53 family also induces p53R2 expression, making p53R2 an important target of p53 for tumor suppression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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