Thermo Fisher Scientific Human Lactoferrin Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Goats were immunized with purified human lactoferrin from human milk. The antiserum was solid-phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient, where an absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Target Information

Lactoferrin (LF) is an iron-binding glycoprotein composed of a single polypeptide chain (~80 kDa) folded into two homologous lobes, each capable of reversibly binding one ferric ion (Fe³⁺). It is found in external fluids such as milk and mucosal secretions, and within secondary granules of neutrophils. LF exhibits diverse biological functions, including:

• Regulation of iron homeostasis
• Host defense against microbial infections
• Anti-inflammatory activity
• Regulation of cellular growth and differentiation
• Protection against cancer development and metastasis

LTF acts as a major first-line defense against microbial infections through iron sequestration and direct bactericidal activity. It can cleave arginine-rich sequences in microbial proteins. Three isoforms (LTF-α, LTF-β, LTF-γ) have been identified, and LTF receptors are expressed in the gastrointestinal tract, macrophages, lymphocytes, neutrophils, platelets, and some bacteria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.