Thermo Fisher Scientific PTPN2 Monoclonal Antibody (T25-AF3B7)

Applications and Tested Dilution

Product Specifications

Product Specific Information

A suggested positive control for this product is Bosc23 cell lysate transfected with Myc/His/TCPTP.

Target Information

T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations.
The shorter 45 kDa isoform is exclusively nuclear in resting cells but redistributes to the cytosol upon stimulation with growth factors and cellular stress. The longer 48 kDa isoform is exclusively found in the endoplasmic reticulum and seems to have distinctly different physiologic substrates from the smaller isoform.
Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types. Knockout mice lacking TC-PTP are born viable but die 3–5 weeks after birth due to erythropoietic and lymphopoietic deficits, indicating a critical role for TC-PTP in bone marrow maturation. TC-PTP dephosphorylates a wide range of phosphoproteins, such as p52 Shc and receptors for EGF, Insulin, and growth hormone.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.