Thermo Fisher Scientific PTPN2 Monoclonal Antibody (T25-AF3B7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

T25-AF3B7

Immunogen

Recombinant human protein purified from E.coli

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2538717

Product Specific Information

A suggested positive control for this product is Bosc23 cell lysate transfected with Myc/His/TCPTP.

Target Information

T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations. The shorter 45 kilodalton isoform is exclusively nuclear in resting cells, but redistrubutes to the cytosol upon stimulation with growth factors and cellular stress. The longer 48 kilodalton isoform is exclusively found in the endoplasmic reticulum 3 and seems to have distinctly different physiologic substrates from the smaller isoform. Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types. Knockout mice lacking TC-PTP are born viable but die 3 to 5 weeks after birth of erythropoietic and lymphopoietic deficits 7, indicating a critical role for TC-PTP in bone marrow maturation. TC-PTP will dephosphorylate a wide range of phosphoproteins, such as p52 Shc 6 and receptors for EGF 1, Insulin 8 and growth hormone.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.