Thermo Fisher Scientific LDHA Monoclonal Antibody (AF14A11)
LDHA 단백질을 검출하기 위한 Thermo Fisher Scientific의 단클론 항체. Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 적합. HeLa 세포를 양성 대조군으로 권장. 연구용으로만 사용 가능.
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Thermo Fisher Scientific LDHA Monoclonal Antibody (AF14A11)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | - |
| Flow Cytometry (Flow) | - | View 1 publication |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | 2 µg | - |
Product Specifications
| Property | Description |
|---|---|
| Published species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AF14A11 |
| Immunogen | Recombinant human protein purified from E.coli (His-LDHA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2538715 |
Product Specific Information
A suggested positive control for this product is HeLa cells.
Target Information
Lactate dehydrogenase (LDH) is an enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+.
In mammals, the enzyme is encoded by three genes: LDHA (muscle form), LDHB (heart form), and LDHC (testis form).
The three human LDHs share 84–89% sequence similarity and 69–75% amino acid identity.
There are five different LDH isoenzymes based on the proportion of M and H chains in the LDH tetrameric structure.
LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of HIF1α.
LDHB is also known to be upregulated in many cancers.
Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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