Thermo Fisher Scientific beta Actin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 24 publications 24 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

View 4 publications 4 publications

Immunoprecipitation (IP)

5 µg/mL

-

Product Specifications

Published species

Dog, Fruit fly, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to N-terminus of human beta-actin if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:beta Actin,uniProtId:P60709-1,ncbiNodeId:9606,antigenRange:1,antigenLength:375,antigenImageFileName:PA5-16914_beta_Actin_P60709-1_Rabbit.svg,antigenImageFileNamePDP:PA5-16914_beta_Actin_P60709-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10976894

Product Specific Information

PA5-16914 targets Actin Beta in IHC (P) and WB applications and shows reactivity with Bovine, Canine, Chicken, Drosophila, Feline, Guinea Pig, Human, Leech, mouse, Ovine, Porcine, Rabbit, and Rat samples.

The PA5-16914 immunogen is synthetic peptide corresponding to N-terminus of human beta-actin.

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.