Thermo Fisher Scientific eNOS Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:500

-

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:70

-

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide derived from C-ternimal of human eNOS if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:eNOS,uniProtId:P29474-1,ncbiNodeId:9606,antigenRange:1203,antigenLength:1203,antigenImageFileName:PA5-16887_eNOS_P29474-1_Rabbit.svg,antigenImageFileNamePDP:PA5-16887_eNOS_P29474-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.081 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984948

Product Specific Information

PA5-16887 targets eNOS in immunohistochemistry (paraffin) applications and shows reactivity with Bovine, Canine, Human, mouse, Porcine, and Rat samples. Suggested positive control is capillary endothelium and regenerative endothelium.

The PA5-16887 immunogen is a synthetic peptide derived from C-ternimal of human eNOS.

Target Information

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.