Thermo Fisher Scientific AHR Polyclonal Antibody

Applications and Tested Dilution

• Application: Immunohistochemistry (Paraffin) [IHC (P)]
• Tested Dilution: 15–20 µg/mL

Product Specifications

Product Specific Information

For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were treated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Percent identity with other species (BLAST analysis):

• Human, Gorilla: 100%
• Gibbon: 94%
• Monkey, Bat, Elephant, Guinea pig: 88%
• Marmoset, Bovine, Hamster: 81%

Target Information

AHR (Aryl Hydrocarbon Receptor) is part of a protein family that includes ARNT (HIF-1 Beta) and Drosophila proteins PER and SIM. It contains an N-terminal PAS domain (~200 amino acids) and is found in various tissues. AHR binds specific DNA enhancer sequences to initiate transcription of cytochrome P-450 (CYPIA1) mRNA.

AHR encodes a ligand-activated transcription factor regulating biological responses to planar aromatic hydrocarbons and xenobiotic-metabolizing enzymes such as cytochrome P450. Known ligands include polycyclic and halogenated aromatic compounds like TCDD. Unlike steroid or thyroid hormone receptors, no physiological ligand is known.

In non-ligand activated cells, AHR complexes with HSP90 in the cytoplasm. Upon agonist binding, AHR transforms into a nuclear DNA-binding form, dissociates from HSP90, and forms a heterodimer with Arnt (AHR nuclear translocator). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.