Thermo Fisher Scientific CD69 Monoclonal Antibody (FN50), Alexa Fluor 660, eBioscience

Thermo Fisher Scientific CD69 Monoclonal Antibody (FN50), Alexa Fluor 660, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test
Publications: -

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 561
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell surface as a disulfide-linked dimer. It is rapidly upregulated upon activation and expressed on lymphocytes, monocytes, and platelets.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometric analysis of stimulated normal human peripheral blood cells.
Recommended usage: 5 µL (0.125 µg) per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test.

Alexa Fluor 660 emits at 690 nm and is suitable for spectral cytometers, multiplexed with APC, Alexa Fluor 647, and Alexa Fluor 700.
Excitation: 662 nm Emission: 690 nm Laser: Red Laser

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains one or two N-linked oligosaccharides and is expressed on activated platelets. In normal peripheral blood, CD69 expression varies across cell populations and is involved in lymphocyte signal transduction. CD69 expression is induced upon T lymphocyte activation and may play a role in proliferation and signaling in NK cells and platelets.
Alternative splicing results in multiple transcript variants.
Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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