Thermo Fisher Scientific Phospho-MST1/MST2 (Thr183, Thr180) Polyclonal Antibody
Phospho-MST1/MST2 (Thr183, Thr180) 인산화 특이 항체로, Hippo 신호 경로 연구에 적합. Western blot에 최적화되어 있으며, Rabbit polyclonal 형식으로 높은 특이성과 재현성 제공. Human 반응성, Mouse/Rat 등 예측 반응성 100%.
- 판매단위
- pk
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human MST1 (phospho Thr183) / MST2 (phospho Thr180) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.54 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736391 |
Product Specific Information
- Positive Control: HeLa (1 µM Staurosporine treatment for 3 hr)
- Predicted Reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Chicken (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
STK3 and STK4 are stress-activated, pro-apoptotic kinases which, following caspase-cleavage, enter the nucleus and induce chromatin condensation followed by DNA fragmentation. These kinases are key components of the Hippo signaling pathway, regulating organ size and tumor suppression by restricting proliferation and promoting apoptosis.
The pathway involves a kinase cascade where STK3/MST2 and STK4/MST1, in complex with SAV1, phosphorylate and activate LATS1/2 with MOB1. This in turn phosphorylates and inactivates YAP1 and WWTR1/TAZ, inhibiting nuclear translocation and regulating genes involved in proliferation, apoptosis, and migration.
STK3/MST2 and STK4/MST1 repress hepatocyte proliferation, prevent activation of liver stem cells, and inhibit tumor formation. They also phosphorylate NKX2-1, NEK2, MOBKL1A, RASSF2, and MOBKL1B, playing roles in centrosome disjunction, RAF1 activation, and ESR1 transcriptional activity modulation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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