Thermo Fisher Scientific p47ING3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:10,000

-

ELISA (ELISA)

1:10,000-1:40,000

-

Immunoprecipitation (IP)

1:200-1:2,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 285-310 of Human ING3 protein (Inhibitor of growth family, member 3). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ING3,uniProtId:Q9NXR8-1,ncbiNodeId:9606,antigenRange:285-310,antigenLength:418,antigenImageFileName:600-101-283_ING3_Q9NXR8-1_Goat.svg,antigenImageFileNamePDP:600-101-283_ING3_Q9NXR8-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Reactivity occurs against human p47 ING3 protein. This sequence only shows homology to isoform 1 for ING3. The sequence is not present in isoforms 2 and 3. However, 100% homology is on record for this protein from human, dog, rat, chimpanzee and orangutan. Cross reactivity with p47 ING3 protein from mouse and chicken sources is also expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p47 ING3 proteins from other sources is not known.

Target Information

The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Two alternatively spliced transcript variants encoding different isoforms have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.