Thermo Fisher Scientific DNER Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

5-15 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse DNER Ala26-His637 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DNER,uniProtId:Q8JZM4-1,ncbiNodeId:10090,antigenRange:26-637,antigenLength:737,antigenImageFileName:PA5-47280_DNER_Q8JZM4-1_Goat.svg,antigenImageFileNamePDP:PA5-47280_DNER_Q8JZM4-1_Goat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2606762

Product Specific Information

In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human DNER is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

DNER (Delta-notch-like EGF-related receptor), also known as Delta-notch-like EGF repeat-containing transmembrane protein, is a neuron-specific, atypical Notch ligand expressed in dendrites and cell bodies of neurons throughout the central nervous system. DNER contains ten extracellular EGF-like domains that are highly homologous to those of the Notch ligand, Delta. In the cerebellum, DNER is predominantly expressed in Purkinje cells. DNER mediates neuron-glia interaction during astrocytogenesis through a direct interaction with Notch 1 at Purkinje cell/Bergmann glia contacts. This interaction activates a Deltex-dependent Notch signaling pathway in Bergmann glia and may regulate Bergmann glial morphogenesis. DNER is crucial for the functional and morphological maturation of Bergmann glia. DNER-knockout mice are characterized by motor discoordination and cerebellum retardation in morphogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.