Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Blue 660-120S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test
Publications: -

Product Specifications

Additional Formats

• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody GAP.A3 reacts with the human major histocompatibility complex (MHC) class I molecule HLA-A3, a member of the HLA-A family.
HLA-A3 exists as two subtypes (HLA-A3.1 and HLA-A3.2), differing at amino acid positions 152 and 156. MHC class I molecules are expressed on all nucleated cells and present intracellular peptides to cytotoxic T lymphocytes.

HLA-A3 is expressed by 15–25% of cancer patients and binds the tumor-associated antigen human telomerase reverse transcriptase (hTERT).

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometry on normal human peripheral blood cells.
Use at 5 µL (0.6 µg) per test (final volume 100 µL; 10⁵–10⁸ cells/test).

Note:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Recommended viability dyes: Invitrogen LIVE/DEAD Fixable Dead Cell stains.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  Add 5 µL per sample or per 100 µL cell suspension (10³–10⁸ cells).

NovaFluor conjugates are based on Phiton™ technology, utilizing nucleic acid dye structures engineered for minimal spillover and spread.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

HLA-A is a class I heavy chain paralogue forming a heterodimer with β2-microglobulin.
These molecules present peptides from the endoplasmic reticulum lumen on nearly all nucleated cells.

• Heavy chain: ~45 kDa, encoded by 8 exons
• Exon 1: Leader peptide
• Exons 2–3: α1, α2 domains (peptide binding)
• Exon 4: α3 domain
• Exon 5: Transmembrane region
• Exons 6–7: Cytoplasmic tail

Polymorphisms in exons 2–3 determine peptide-binding specificity.
HLA typing is routinely performed for bone marrow and kidney transplantation.
Hundreds of HLA-A alleles have been described.

HLA and MHC antibodies are essential in Immunopeptidomics, enabling neoantigen identification and characterization via high-performance LC-MS/MS.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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