Thermo Fisher Scientific H2BK20ac Recombinant Rabbit Monoclonal Antibody (PS01-46)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:50

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

PS01-46

Immunogen

Synthetic peptide surrounding acetylated Lys5 of human histone H2B protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H2BK20ac,uniProtId:O60814-1,ncbiNodeId:9606,antigenRange:5,antigenLength:126,antigenImageFileName:MA5-50787_H2BK20ac_O60814-1_Rabbit.svg,antigenImageFileNamePDP:MA5-50787_H2BK20ac_O60814-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3094434

Product Specific Information

Positive control: Hela treated with 500 ng/mL TSA for 4 hours whole cell lysate, human colon carcinoma tissue, human skin tissue, mouse skin tissue, rat skin tissue, Hela, NIH/3T3.

Predicted band size: 14 kDa

Subcellular Location: Nucleus, Chromosome.

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The nucleosomes wrap further and compact DNA into chromotin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transciption regulation, DNA repair, DNA replication, and chromosomal stability. H2B has a broad antibacterial activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.