Thermo Fisher Scientific H3R2me1 Recombinant Rabbit Monoclonal Antibody (ST0427)
H3R2me1 표적의 재조합 토끼 단일클론 항체로, Western blot, IHC, ICC/IF에 사용 가능. HEK293 발현 시스템 기반, Protein A 정제, 고순도 액상 형태. 인간 및 생쥐 시료에 반응하며, 핵 내 히스톤 H3 인식. 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ST0427 |
| Immunogen | Synthetic peptide within human histone H3 amino acids 1–49 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2911576 |
Product Specific Information
- Positive Control: NIH/3T3, PC-3M, HepG2
- Subcellular Location: Nucleus, Chromosome
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translationally, histones are modified in various ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the nucleosome core and undergoes several types of post-translational modification, such as methylation, acetylation, and phosphorylation, influencing cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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