Thermo Fisher Scientific Emerin Monoclonal Antibody (EMD, 2168)
Emerin 단백질을 표적으로 하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IHC, Peptide Array에 사용 가능. 인간 시료 반응성, 마우스 IgG2b 형식, 고순도 Protein A/G 정제. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | EMD, 2168 |
| Immunogen | Recombinant human Emerin protein fragment (around aa 56–167) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Xeroderma pigmentosum type G (XPG) is a human genetic disease exhibiting extreme sensitivity to sunlight.
The XPG protein, a member of the flap endonuclease 1 (FEN-1) structure-specific DNA repair endonuclease family, is essential for DNA repair of UV-induced DNA damages.
Human XPG nuclease makes the 3′ incision during nucleotide excision repair of DNA and cleaves model DNA bubble structures near the junction of unpaired DNA with a duplex region.
A 29-amino acid region of human XPG (residues 981–1009) contains the PCNA binding activity. A conserved arginine (Arg992) is crucial for this binding.
Replication Protein A (RPA) binds specifically and directly to two excision repair proteins, XPA and XPG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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