Thermo Fisher Scientific Emerin Monoclonal Antibody (EMD, 2167)
Thermo Fisher Scientific의 Emerin 단클론 항체(EMD, 2167)는 인간 Emerin 단백질을 인식하는 Mouse IgG1 항체로, WB, IHC, ICC 등 다양한 응용에 적합합니다. 고순도 Protein A/G 정제, 보존제 무첨가 PBS 용액 형태로 제공되며 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–3 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | EMD, 2167 |
| Immunogen | Recombinant human Emerin protein fragment (around aa 56–167) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Xeroderma pigmentosum type G (XPG) is a human genetic disease exhibiting extreme sensitivity to sunlight. The XPG protein, a member of the flap endonuclease 1 (FEN-1) structure-specific DNA repair endonuclease family, is essential for DNA repair of solar UV-induced DNA damages. Human XPG nuclease makes the 3′ incision during nucleotide excision repair of DNA, cleaving model DNA bubble structures near the junction of unpaired DNA with a duplex region. A 29-amino acid region of human XPG (residues 981–1009) contains PCNA binding activity, with Arg992 being crucial for this interaction. Replication Protein A (RPA) binds specifically and directly to two excision repair proteins, XPA and XPG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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