Thermo Fisher Scientific Presenilin-1 Polyclonal Antibody, Atto 488

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

1:60

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)RDSHLGPHRSTPESR, corresponding to amino acid residues 345-359 of rat Psen1, 3rd cytoplasmic loop (at the Psen1 CTF subunit) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Presenilin 1,uniProtId:P97887-1,ncbiNodeId:10114,antigenRange:345-359,antigenLength:468,antigenImageFileName:AIP-011-AG-50UL_Presenilin_1_P97887-1_Rabbit.svg,antigenImageFileNamePDP:AIP-011-AG-50UL_Presenilin_1_P97887-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Atto™ 488 Atto™ 488 Atto™ 488

Excitation/Emission Max

501/523 nm

Form

Lyophilized

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

Presenilin1 was initially identified a marker of susceptibility to early-onset Alzheimers disease. In addition to PEN2, nicastrin and APH-1, Presenilin1 forms the -gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimers disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, -gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and -gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and -gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer`s disease. Multiple isoforms of presenilin1 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.