Thermo Fisher Scientific p115-RhoGEF Polyclonal Antibody
Rabbit polyclonal antibody recognizing human p115-RhoGEF. Validated for WB and IP applications. Targets residues 275–325 of ARHGEF1 protein. Supplied as liquid at 0.2 mg/mL, stored at 4°C. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 275 and 325 of human 115 kDa Rho guanine nucleotide exchange factor (ARHGEF1, UniProt ID: Q92888-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from the date of receipt.
Target Information
The Ras superfamily of GTPases, subdivided into Ras, Rho/Rac, Sar, Rab, ARF, and Ran subfamilies, regulates various cellular processes such as cytoskeletal rearrangement, nuclear signaling, and cell growth. These GTPases act as molecular switches, alternating between an active GTP-bound and inactive GDP-bound state. Activation is catalyzed by guanine nucleotide exchange factors (GEFs).
Dbl-related proteins, including FGD1, Lsc, RhoGEF p115, Lfc, Lbc, and Brx, share structural similarities and GEF activity toward specific Ras family members. Lsc, Lbc, and Lfc show exchange activity toward Rho family GTPases. RhoGEF p115 catalyzes GEF activity for Rho but not for Rac, Cdc42, or Ras GTPases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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